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luciferase labeled raji cells  (ATCC)


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    Structured Review

    ATCC luciferase labeled raji cells
    (A) CAR expression in activated human T cells 24-hours after treatment with unconjugated LNP–CAR mRNA or aCD7/tLNP-CAR mRNA at doses of 0.01, 0.1, or 1 μg mRNA per million cells. (B and C) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with unconjugated LNP-CAR mRNA. (D and E) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with aCD7/tLNP-CAR mRNA. (F) Experimental scheme for evaluating the cytolytic activity of CAR T cells against target cells. (G) Representative images showing Cytotox Green fluorescence indicating <t>Raji</t> cell death in co-cultures with T cells treated with unmodified LNP-CAR or aCD7/tLNP-CAR at effector-to-target (E: T) ratios of 1:1, 2:1, 5:1, and 10:1. (H) Quantification of Cytotox Green fluorescence shown in (G). (I) Quantification of Raji cell viability in co-culture based on luciferase activity. n = 2 biological replicates. Data are shown as mean ± SD. Scale bar = 100 μm.
    Luciferase Labeled Raji Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase labeled raji cells/product/ATCC
    Average 94 stars, based on 7 article reviews
    luciferase labeled raji cells - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Rapid receptor internalization potentiates CD7-targeted lipid nanoparticles for efficient mRNA delivery to T cells and in vivo CAR T-cell engineering"

    Article Title: Rapid receptor internalization potentiates CD7-targeted lipid nanoparticles for efficient mRNA delivery to T cells and in vivo CAR T-cell engineering

    Journal: bioRxiv

    doi: 10.64898/2026.01.23.701374

    (A) CAR expression in activated human T cells 24-hours after treatment with unconjugated LNP–CAR mRNA or aCD7/tLNP-CAR mRNA at doses of 0.01, 0.1, or 1 μg mRNA per million cells. (B and C) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with unconjugated LNP-CAR mRNA. (D and E) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with aCD7/tLNP-CAR mRNA. (F) Experimental scheme for evaluating the cytolytic activity of CAR T cells against target cells. (G) Representative images showing Cytotox Green fluorescence indicating Raji cell death in co-cultures with T cells treated with unmodified LNP-CAR or aCD7/tLNP-CAR at effector-to-target (E: T) ratios of 1:1, 2:1, 5:1, and 10:1. (H) Quantification of Cytotox Green fluorescence shown in (G). (I) Quantification of Raji cell viability in co-culture based on luciferase activity. n = 2 biological replicates. Data are shown as mean ± SD. Scale bar = 100 μm.
    Figure Legend Snippet: (A) CAR expression in activated human T cells 24-hours after treatment with unconjugated LNP–CAR mRNA or aCD7/tLNP-CAR mRNA at doses of 0.01, 0.1, or 1 μg mRNA per million cells. (B and C) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with unconjugated LNP-CAR mRNA. (D and E) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with aCD7/tLNP-CAR mRNA. (F) Experimental scheme for evaluating the cytolytic activity of CAR T cells against target cells. (G) Representative images showing Cytotox Green fluorescence indicating Raji cell death in co-cultures with T cells treated with unmodified LNP-CAR or aCD7/tLNP-CAR at effector-to-target (E: T) ratios of 1:1, 2:1, 5:1, and 10:1. (H) Quantification of Cytotox Green fluorescence shown in (G). (I) Quantification of Raji cell viability in co-culture based on luciferase activity. n = 2 biological replicates. Data are shown as mean ± SD. Scale bar = 100 μm.

    Techniques Used: Expressing, Activity Assay, Fluorescence, Co-Culture Assay, Luciferase



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    luciferase labeled raji cells  (ATCC)
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    ATCC luciferase labeled raji cells
    (A) CAR expression in activated human T cells 24-hours after treatment with unconjugated LNP–CAR mRNA or aCD7/tLNP-CAR mRNA at doses of 0.01, 0.1, or 1 μg mRNA per million cells. (B and C) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with unconjugated LNP-CAR mRNA. (D and E) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with aCD7/tLNP-CAR mRNA. (F) Experimental scheme for evaluating the cytolytic activity of CAR T cells against target cells. (G) Representative images showing Cytotox Green fluorescence indicating <t>Raji</t> cell death in co-cultures with T cells treated with unmodified LNP-CAR or aCD7/tLNP-CAR at effector-to-target (E: T) ratios of 1:1, 2:1, 5:1, and 10:1. (H) Quantification of Cytotox Green fluorescence shown in (G). (I) Quantification of Raji cell viability in co-culture based on luciferase activity. n = 2 biological replicates. Data are shown as mean ± SD. Scale bar = 100 μm.
    Luciferase Labeled Raji Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) CAR expression in activated human T cells 24-hours after treatment with unconjugated LNP–CAR mRNA or aCD7/tLNP-CAR mRNA at doses of 0.01, 0.1, or 1 μg mRNA per million cells. (B and C) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with unconjugated LNP-CAR mRNA. (D and E) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with aCD7/tLNP-CAR mRNA. (F) Experimental scheme for evaluating the cytolytic activity of CAR T cells against target cells. (G) Representative images showing Cytotox Green fluorescence indicating <t>Raji</t> cell death in co-cultures with T cells treated with unmodified LNP-CAR or aCD7/tLNP-CAR at effector-to-target (E: T) ratios of 1:1, 2:1, 5:1, and 10:1. (H) Quantification of Cytotox Green fluorescence shown in (G). (I) Quantification of Raji cell viability in co-culture based on luciferase activity. n = 2 biological replicates. Data are shown as mean ± SD. Scale bar = 100 μm.
    Raji Luc2, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    raji luc2 cell culture  (ATCC)
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    ATCC raji luc2 cell culture
    (A) CAR expression in activated human T cells 24-hours after treatment with unconjugated LNP–CAR mRNA or aCD7/tLNP-CAR mRNA at doses of 0.01, 0.1, or 1 μg mRNA per million cells. (B and C) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with unconjugated LNP-CAR mRNA. (D and E) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with aCD7/tLNP-CAR mRNA. (F) Experimental scheme for evaluating the cytolytic activity of CAR T cells against target cells. (G) Representative images showing Cytotox Green fluorescence indicating <t>Raji</t> cell death in co-cultures with T cells treated with unmodified LNP-CAR or aCD7/tLNP-CAR at effector-to-target (E: T) ratios of 1:1, 2:1, 5:1, and 10:1. (H) Quantification of Cytotox Green fluorescence shown in (G). (I) Quantification of Raji cell viability in co-culture based on luciferase activity. n = 2 biological replicates. Data are shown as mean ± SD. Scale bar = 100 μm.
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    (A) CAR expression in activated human T cells 24-hours after treatment with unconjugated LNP–CAR mRNA or aCD7/tLNP-CAR mRNA at doses of 0.01, 0.1, or 1 μg mRNA per million cells. (B and C) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with unconjugated LNP-CAR mRNA. (D and E) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with aCD7/tLNP-CAR mRNA. (F) Experimental scheme for evaluating the cytolytic activity of CAR T cells against target cells. (G) Representative images showing Cytotox Green fluorescence indicating <t>Raji</t> cell death in co-cultures with T cells treated with unmodified LNP-CAR or aCD7/tLNP-CAR at effector-to-target (E: T) ratios of 1:1, 2:1, 5:1, and 10:1. (H) Quantification of Cytotox Green fluorescence shown in (G). (I) Quantification of Raji cell viability in co-culture based on luciferase activity. n = 2 biological replicates. Data are shown as mean ± SD. Scale bar = 100 μm.
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    raji luciferase  (ATCC)
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    ATCC raji luciferase
    (A) CAR expression in activated human T cells 24-hours after treatment with unconjugated LNP–CAR mRNA or aCD7/tLNP-CAR mRNA at doses of 0.01, 0.1, or 1 μg mRNA per million cells. (B and C) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with unconjugated LNP-CAR mRNA. (D and E) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with aCD7/tLNP-CAR mRNA. (F) Experimental scheme for evaluating the cytolytic activity of CAR T cells against target cells. (G) Representative images showing Cytotox Green fluorescence indicating <t>Raji</t> cell death in co-cultures with T cells treated with unmodified LNP-CAR or aCD7/tLNP-CAR at effector-to-target (E: T) ratios of 1:1, 2:1, 5:1, and 10:1. (H) Quantification of Cytotox Green fluorescence shown in (G). (I) Quantification of Raji cell viability in co-culture based on luciferase activity. n = 2 biological replicates. Data are shown as mean ± SD. Scale bar = 100 μm.
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    raji luc cells  (ATCC)
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    ATCC raji luc cells
    (A) CAR expression in activated human T cells 24-hours after treatment with unconjugated LNP–CAR mRNA or aCD7/tLNP-CAR mRNA at doses of 0.01, 0.1, or 1 μg mRNA per million cells. (B and C) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with unconjugated LNP-CAR mRNA. (D and E) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with aCD7/tLNP-CAR mRNA. (F) Experimental scheme for evaluating the cytolytic activity of CAR T cells against target cells. (G) Representative images showing Cytotox Green fluorescence indicating <t>Raji</t> cell death in co-cultures with T cells treated with unmodified LNP-CAR or aCD7/tLNP-CAR at effector-to-target (E: T) ratios of 1:1, 2:1, 5:1, and 10:1. (H) Quantification of Cytotox Green fluorescence shown in (G). (I) Quantification of Raji cell viability in co-culture based on luciferase activity. n = 2 biological replicates. Data are shown as mean ± SD. Scale bar = 100 μm.
    Raji Luc Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) CAR expression in activated human T cells 24-hours after treatment with unconjugated LNP–CAR mRNA or aCD7/tLNP-CAR mRNA at doses of 0.01, 0.1, or 1 μg mRNA per million cells. (B and C) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with unconjugated LNP-CAR mRNA. (D and E) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with aCD7/tLNP-CAR mRNA. (F) Experimental scheme for evaluating the cytolytic activity of CAR T cells against target cells. (G) Representative images showing Cytotox Green fluorescence indicating Raji cell death in co-cultures with T cells treated with unmodified LNP-CAR or aCD7/tLNP-CAR at effector-to-target (E: T) ratios of 1:1, 2:1, 5:1, and 10:1. (H) Quantification of Cytotox Green fluorescence shown in (G). (I) Quantification of Raji cell viability in co-culture based on luciferase activity. n = 2 biological replicates. Data are shown as mean ± SD. Scale bar = 100 μm.

    Journal: bioRxiv

    Article Title: Rapid receptor internalization potentiates CD7-targeted lipid nanoparticles for efficient mRNA delivery to T cells and in vivo CAR T-cell engineering

    doi: 10.64898/2026.01.23.701374

    Figure Lengend Snippet: (A) CAR expression in activated human T cells 24-hours after treatment with unconjugated LNP–CAR mRNA or aCD7/tLNP-CAR mRNA at doses of 0.01, 0.1, or 1 μg mRNA per million cells. (B and C) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with unconjugated LNP-CAR mRNA. (D and E) Quantification of CAR expression in CD4⁺ and CD8⁺ T cells treated with aCD7/tLNP-CAR mRNA. (F) Experimental scheme for evaluating the cytolytic activity of CAR T cells against target cells. (G) Representative images showing Cytotox Green fluorescence indicating Raji cell death in co-cultures with T cells treated with unmodified LNP-CAR or aCD7/tLNP-CAR at effector-to-target (E: T) ratios of 1:1, 2:1, 5:1, and 10:1. (H) Quantification of Cytotox Green fluorescence shown in (G). (I) Quantification of Raji cell viability in co-culture based on luciferase activity. n = 2 biological replicates. Data are shown as mean ± SD. Scale bar = 100 μm.

    Article Snippet: To assess cytotoxic activity, CAR T cells were then co-cultured with CD20-expressing, luciferase-labeled Raji cells (ATCC, cat# CCL-86-LUC2) at effector-to-target ratios of 1:1, 2:1, 5:1, and 10:1.

    Techniques: Expressing, Activity Assay, Fluorescence, Co-Culture Assay, Luciferase